2:00 – 7:00 PM Registration in the Stuyvesant Ballroom Foyer
5:30– 5:40 PM Welcome by Co-chairs,
Stuyvesant Ballroom
Ethel Cesarman & Laurie Krug
5:40 – 6:00 PM Reflections on KSHV @ 25 years
Yuan Chang and Patrick Moore
6:00
– 7:30 PM Session I. Hot topics
Stuyvesant Ballroom
Session Chairs
Yuan Chang, University of Pittsburgh, United States
Patrick Moore, University of Pittsburgh, United States
Presentations
6:00 – 6:15 PM (01) Kaposi
Sarcoma in Whole KSHV Genome Transgenic Mice
Dirk Dittmer,
University of North Carolina at Chapel Hill,
Chapel Hill, United States
6:15 – 6:30 PM (02) Employing
the Virus Alone to Diagnose the Cancer:
Quantification of Lesional KSHV DNA for the Diagnosis of Kaposi Sarcoma
in Africa
Jeffrey Martin, University of California,
San Francisco, San Francisco, United States
6:30 – 6:45 PM (03) Lymphatic
endothelial cells support a spontaneous KSHV lytic infection program through
activities of PROX1 and SOX18 transcription factors
Paivi Ojala,
University of Helsinki, University of Helsinki, Finland
6:45 – 7:00 PM (04) Enhanced
Xrn1 activity broadly represses RNA polymerase II occupancy at mammalian but
not viral promoters during gammaherpesvirus infection
Britt Glaunsinger,
University Of California Berkeley/HHMI,
Berkeley, United
States
7:00 – 7:15 PM (05) Discovery
and Function of enhancer RNAs associated with KSHV infection
Angela Park, USC, Los Angeles, United
States
7:15 – 7:30 PM (06) Novel Role
of the Innate Immune DNA Sensor IFI16 as a Major Epigenetic Modulator of KSHV
Gene Expression
Arunava Roy, Morsani College of Medicine,
University of South Florida, Tampa, Tampa,
United States
7:30 –
9:30 PM Welcome Reception
Morgan Ballroom A-D
Session Chairs
Jennifer Corcoran, University of Calgary, Canada
Neelam Sharma-Walia, RFUMS, United States
Presentations
8:30 – 8:45 AM (07) EphA7
Functions as a Receptor on BJAB Cells for cell-to-cell Transmission of KSHV and
cell-free Infection by the Related Rhesus Monkey Rhadinovirus (RRV)
Anna Großkopf,
Deutsches Primatenzentrum – Leibniz-Institut
für Primatenforschung GmbH, Göttingen,
Germany
8:45 – 9:00 AM (08) Kaposi
Sarcoma-associated Herpesvirus Glycoprotein H is Indispensable for Infection of
Epithelial, Endothelial, and Fibroblast Cell Types
Javier Ogembo, Beckman Research
Institute of City Of Hope, Duarte,
United States
9:00 – 9:15 AM (09) RecQL
helicase unwinds the G-quadruplexes in the origin of lytic DNA replication to
initiate replication
Subhash Verma, University of Nevada, Reno
School of Medicine, Reno, United States
9:15 – 9:30 AM (10) Assessing
the Role of the Anaphase Promoting Complex (APC/C) and the Impact of the APC/C
Induced Re-replication in the KSHV Lytic Cycle
Endrit Elbasani, University of Helsinki,
Helsinki, Finland
9:30 – 9:45 AM (11) KSHV hijacks
CAD-mediated RelA deamidation to Promote Glycolysis and Cell Proliferation
Jun Zhao,
Herman Ostrow School of Dentistry, University
of Southern California, Los Angeles,
United States
9:45 – 10:00 AM (12) HACE1, an E3
Ubiquitin-Protein Lligase, Mitigates KSHV Infection Induced Oxidative Stress by
Promoting Nrf2 Activity
Bala Chandran,
Morsani College Of Medicine, University Of
South Florida, Tampa, United States
10:00 – 10:15 AM (13) Protein
Deamidation Mediated Metabolic Reprogramming During KSHV Lytic Replication
Mao Tian,
University of Southern California, LOS ANGELES, United
States
10:15 – 10:30 AM (14) LXRα
activation impedes herpesvirus egress
Carolyn-Ann Robinson, University of Calgary,
Calgary, Canada
10:30 – 11:00 AM Coffee Break, Set up Posters, Visit the
Exhibits
Morgan Ballroom A-C
11:00
AM –1:00 PM Session III.
Processes of Virus Latency
Stuyvesant Ballroom
Session Chairs
JJ Miranda, Barnard College, Columbia University, United States
Angus Wilson, New York University School of Medicine, United States
Presentations
11:00 – 11:15 AM (15) LANA
oligomerization and viral genome maintenance during KSHV latency
Paul Lieberman,
Wistar Institute, Philadelphia, United
States
11:15 – 11:30 AM (16) Elucidating
the Roles of Caspase Cleavage sites in KSHV LANA using Recombinant KSHV
David Davis,
NIH/NCI, Bethesda, United States
11:30 – 11:45 AM (17) Most KSHV
LANA Internal Repeat Elements are Dspensable for in vivo Latency in Chimeric
Virus
Kenneth Kaye, Brigham And Women’s
Hospital, Harvard Medical School, Boston,
United States
11:45 – 12:00 PM (18) Spatiotemporal
Control of KSHV Chromatin Assembly During De Novo Infection
Thomas Günther, Heinrich Pette
Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany
12:00 – 12:15 PM (19) Identification
of Novel Epigenetic Factors Controlling the Establishment of KSHV Latency Using
an Epigenetic Factor siRNA Screen
GOPAL NAIK NENAVATH, University of Florida,
Gainesville, United States
12:15 – 12:30 PM (20) A Temporal
Analysis of Host Chromatin Changes and Transcriptional Responses Induced by
Latent Kaposi’s Sarcoma-associated Herpesvirus Infection
Jacqueline Fröhlich, Heinrich Pette
Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany
12:30 – 12:45 PM (21) A
CRISPR/Cas9 Screen in Endothelial Cells Reveals Mitochondrial Translation as an
Essential Process for Latent KSHV Infection
Michael Lagunoff, University Of
Washington, Seattle, United States
12:45 – 1:00 PM (22) Lytic
Replication and Reactivation from B Cells Is Not Required for Murine
Gammaherpesvirus Latency Establishment and Long-term Maintenance
Craig Forrest,
University of Arkansas for Medical Sciences,
Little Rock, United States
1:00– 2:00 PM Group Lunch in the Poster &
Exhibit Room
Morgan Ballroom A-C
2:00 – 3:00 PM Poster Session Part 1: Even-Numbered Posters Presented
Morgan Ballroom A-C
Session Chairs
Blossom Damania, University of North Carolina at Chapel Hill, United States
Myung-Shin Lee, Eulji University School of Medicine, Republic of Korea
Presentations
3:00 – 3:15 PM (23) Inhibition
of the DNA damage response by a gammaherpesviral tegument protein
Armin Ensser,
Institute for Clinical and Molecular
Virology, University Hospital Erlangen, Erlangen,
Germany
3:15 – 3:30 PM (24) Functional proteomic
analysis and CRISPR Cas9-targeting of KSHV genomes identifies novel KSHV
targets in human endothelial cells
Ildar Gabaev,
University of Cambridge, Cambridge, United
Kingdom
3:30 – 3:45 PM (25) vIRFs are
dispensable for KSHV production, but contribute to the inhibition of the Type I
Interferon pathway to promote the KSHV lytic cycle
Zsolt Toth,
University Of Florida, Gainesville, United
States
3:45 – 4:00 PM (26) Caspase-8
suppresses STING mediated Type I Interferon induction during KSHV lytic
replication
Tate Tabtieng,
Tufts University School of Medicine, Boston, United
States
4:00 – 4:15 PM (27) Physical and
Functional Targeting of Ubiquitin-Specific Protease 7 by Human Herpesvirus 8
Viral Interferon Regulatory Factor-2
John Nicholas,
Johns Hopkins University, Baltimore, United
States
4:15 – 4:30 PM (28) Eukaryotic
DExD/H box helicases DDX24 and DDX49 modulate host innate immune response via
recognizing viral mRNAs to inhibit Lytic Replication of Kaposi’s Sarcoma
Associated Herpesvirus
Yuan Hong,
University of Florida, Gainesville, United
States
4:30 – 5:00 PM Coffee Break, Visit the Posters &
Exhibits
Morgan Ballroom A-C
5:00
– 7:00 PM Session V. Virus
Pathogenesis and Cancer
Stuyvesant Ballroom
Session Chairs
Ashlee Moses, Oregon Health & Science University, United States
Meir Shamay, Bar-Ilan University, Israel
Presentations
5:00 – 5:15 PM (29) CRISPR-Cas9
Screening of KSHV-Transformed Cells Identifies XPO1 as a Vulnerable Target of Cancer
Cells by Inducing p62 SQSTM1-Mediated p53 Activation in PML Bodies
Shou-Jiang Gao, University of Pittsburgh,
Pittsburgh, United States
5:15 – 5:30 PM (30) Kaposi’s
Sarcoma Herpesvirus Is Associated with Osteosarcoma
Yan Yuan,
University of Pennsylvania, Philadelphia, United
States
5:30 – 5:45 PM (31) Viral
manipulation of a novel mechanoresponsive signaling axis disassembles
processing bodies to alter the tumour microenvironment
Jennifer Corcoran, University of Calgary,
Calgary, Canada
5:45 – 6:00 PM (32) The cellular
genome of Kaposi sarcoma
Warren Phipps, Fred Hutchinson Cancer Research Center, Seattle, United
States
6:00 – 6:15 PM (33) PDGFRA
Defines the Mesenchymal Stem Cell Kaposi’s Sarcoma Progenitors by Enabling KSHV
Oncogenesis in an Angiogenic Environment
Enrique Mesri, University of Miami Miller
School of Medicine, Miami, United States
6:15 – 6:30 PM (34) Histamine
Promotes the Oncogenic Capacity of KSHV-latently Infected Primary Endothelial
Cells.
Christine King, SUNY Upstate Medical
University, Syracuse, United States
6:30 – 6:45 PM (35) The Role of
Wilms’ Tumor 1 (WT1) in Kaposi Sarcoma Herpesvirus Oncogenesis
Ayana Morales,
Weill Cornell Medicine, New York, United
States
6:45 – 7:00 PM (36) KSHV vIRF1 upregulates
CDCP1 to promote cell invasion and angiogenesis by targeting LEF1 and CD82
Wan Li,
Nanjing Medical University, Nanjing, China
Tuesday, July 2, 2019
Session Chairs
Mandy Muller, University of Massachusetts, Amherst, United States
Cyprian Rossetto, University of Nevada, Reno, United States
Presentations
8:30 – 8:45 AM (37) Single Cell Analysis
Reveals Heterogeneous Lytic Cycle Progression in MHV68
Linda Van
Dyk, University of Colorado Anschutz
Medical Campus, Aurora, United States
8:45 – 9:00 AM (38) KSHV ORF57
Regulates the Expression of Host RNAs by Direct Binding and Contributes to Viral
Pathogenesis
Beatriz Alvarado Hernandez, National
Cancer Institute, Frederick, United States
9:00 – 9:15 AM (39) KS-associated
herpesvirus Rta and cellular POU proteins regulate DNA binding of the Notch
signaling protein RBP-Jk/CSL broadly to the virus genome
David Lukac,
Rutgers Univ/Rutgers NJ Medical School,
Newark, United
States
9:15 – 9:30 AM (40) Lytic
Reactivation of KSHV is Associated with Major Alterations of the Nucleolar
Morphology and Composition
Ronit Sarid,
Bar Ilan University, Ramat Gan, Israel
9:30 – 9:45 AM (41) The
Autophagy Regulator HDAC6 Coordinates Successful KSHV Reactivation
Helena Mello, Rutgers University,
Newark, United
States
9:45 – 10:00 AM (42) PROX1mal
Interactions with Viral and Cellular Factors Regulating the KSHV Lytic
Replication
Krista Tuohinto, University of Helsinki,
Helsinki, Finland
10:00 – 10:15 AM (43) Activation of phospholipase Cg1 by the KSHV K15 protein: molecular mechanism and novel antiviral target? Thomas Schulz, Hannover Medical School, Hannover, Germany
10:15 – 10:30 AM (44) Phosphatase
PP2A promotes dephosphorylation of RTA to inhibit KSHV lytic reactivation
Lianghui Dong, Wuhan University, Wuhan, China
10:30
– 11:00 AM Coffee Break – Visit the
Posters & Exhibits
Morgan Ballroom A-C
Session Chairs
Marta Gaglia, Tufts University School of Medicine, United States
Fanxiu Zhu, Florida State University, United States
Presentations
11:00 – 11:15 AM (45) KSHV encodes
a mimic of the tumor suppressive miR-15/16 family of miRNAs
Eva Gottwein,
Northwestern University, Chicago, United
States
11:15 – 11:30 AM (46) Suppression
of mTORC1 inhibitor CASTOR1 by oncogenic KSHV-encoded microRNAs promotes cell
proliferation and growth transformation
Tingting Li, University of Pittsburgh,
PITTSBURGH, United States
11:30 – 11:45 AM (47) Integration
of Ribonomics Analysis and Phenotypic Assays Reveals Connections Between KSHV
miRNAs, Targets, and Phenotypes
Lauren Gay, University of Florida,
Gainesville, United States
11:45 – 12:00 PM (48) Co-expression
of KSHV Mimics of Cellular miR-155 and miR-23 is Essential in Primary Effusion
Lymphoma Cell Lines
Mark Manzano,
Northwestern University, Chicago, United
States
12:00 – 12:15 PM (49) Understanding
the KSHV miRNA Target Network
Takanobu Tagawa, National Cancer Institute,
Bethesda, United
States
12:15 – 12:30 PM (50) Kaposi’s sarcoma-associated
herpesvirus latency-associated nuclear antigen deregulates MCL1 expression by
targeting the cellular ubiquitin E3 ligase FBW7
Hye-Ra Lee, Korea University, Sejong, Korea,
Republic of
12:30 – 12:45 PM (51) Human
Herpesvirus 8 vIRF-1 Interacts with the Autophagy-Related Mitochondrial
Elongation Factor EF-Tu
CHANG-YONG CHOI, Johns Hopkins University
School of Medicine, Baltimore, United States
12:45 – 01:00 PM (52) The KSHV K1
viral oncoprotein-mediated regulation of proline metabolism for tumorigenesis
Un Yung Choi, University of Southern
California, Los Angeles, United States
1:00 – 2:00 PM Lunch on Own – Opportunity to Network at Nearby Restaurants
1:00 – 3:00 PM Poster Session Part 2: Odd-Numbered Posters Presented
Morgan Ballroom A-C
Session Chairs
Young Bong Choi, Johns Hopkins School of Medicine, United States
Jennifer Totonchy, Chapman University School of Pharmacy, United States
Presentations
3:00 – 3:15 PM (53) KSHV
Activates Unfolded Protein Response Sensors but Suppresses Downstream
Transcriptional Responses to Support Lytic Replication
Craig McCormick,
Dalhousie University, Halifax, Canada
3:15 – 3:30 PM (54) Viral
Factors Influencing Plasmablast Differentiation During Ex-Vivo KSHV Infection
Romina Nabiee, Chapman University School
of Pharmacy, Irvine, United States
3:30 – 3:45 PM (55) Kaposi’s Sarcoma-associated
Herpesvirus stably transforms peripheral B cells and drives them towards
lymphomagenesis
Mitch Hayes,
University of Wisconsin-Madison, Madison, United
States
3:45 – 4:00 PM (56) A
Gammaherpesvirus MicroRNA Targets and Represses Ewing Sarcoma RNA Binding
Protein 1 (EWSR1) to Promote Latent Infection of Germinal Center B Cells
Yiping Wang, University of Florida,
Gainesville, United States
4:00 – 4:15 PM (57) Herpesviral
infection induces a germline-specific transcriptional program mimicking zygotic
gene activation
Florian Full, Institute for Clinical and
Molecular Virology, University Hospital Erlangen, Erlangen, Germany
4:15 – 4:30 PM (58) KSHV
Requires vCyc to Overcome Replicative Senescence in Primary Human Lymphatic
Endothelial Cells
Terri DiMaio,
University of Washington, Seattle, United
States
4:30 – 4:45 PM (59) KSHV
Infection Induces Neuroendocrine Genes Expression in Endothelial Cells Which
Are Involved in the Regulation of Virus-Induced Cell Proliferation
Bala Chandran,
Morsani College Of Medicine, University Of
South Florida, Tampa, United States
4:45 – 5:00 PM (60) Epstein-Barr
virus globally reprograms host 3D genome architecture to achieve immortal
growth.
Bo Zhao,
Brigham and Women’s Hospital, Boston, United
States
7:00 –
10:30 PM Dinner Cruise
Pier 81, World Yacht Duchess Cruise
Transportation to/from dinner cruise
Participants are on their own getting to the Pier for the KSHV private dinner cruise on the World Yacht Duchess, docked at Pier 81 at 12th Ave & W 41st St (see page 8 for detailed instructions).
Boarding is from 7:00 to 7:30 PM, the boat will leave the harbor promptly at 7:30 PM. Food and drinks will be available during boarding time as well. Please bring your registration badge for admittance (but please do not wear your badges on the way to the pier). Please bring a jacket as it might be cool on the water at sunset. See page 8 for directions to the Pier.
At the conclusion of the cruise at 10:30 PM, there will be three rented charter buses to bring participants back to the New York Marriott East Side.
8:30 – 10:45 AM Session IX. Virus Pathogenesis &
Immunity
Stuyvesant Ballroom
Session Chairs
Zhiqiang Qin, University of Arkansas for Medical Sciences, United States
Noula Shembade, University of Miami, United States
Presentations
8:30 – 8:45 (61) Regulation
of Herpesvirus Infection Through PPAR-alpha regulation of Oxidative Stress and
Inflammatory Responses
Lili Tao,
UT Southwestern Medical Center, Dallas, United
States
8:45 – 9:00 (62) Rhesus
Rhadinovirus (RRV)-encoded microRNAs Affect Immune Responses and Viral
Reactivation in vivo
Scott Wong,
OHSU West Campus, Beaverton, United
States
9:00 – 9:15 (63) Triggering
Innate Immunity Pathways during Herpesvirus Infection with
25-hydroxycholesterol
Anna Serquina,
National Cancer Institute, Bethesda, United
States
9:15 – 9:30 (64) Immunological
factors influencing susceptibility of tonsil-derived B lymphocytes to KSHV
infection
Jennifer Totonchy, Chapman University
School Of Pharmacy, Irvine, United States
9:30 – 9:45 (65) A
Recombinant Gamma-2 Herpesvirus Deleted of Glycoprotein L Establishes
Persistent Infection of Rhesus Macaques and Elicits Conventional T Cell
Responses
Alexander Hahn, Deutsches Primatenzentrum –
Leibniz-Institut für Primatenforschung, Nachwuchsgruppe Herpesviren, Göttingen, Germany
9:45 – 10:00 (66) A
Replication Defective Gammaherpesvirus Vaccine that is Safe in Immunodeficient
Mice Protects Against Wild-Type Virus Replication in Immune Competent Mice
Laurie Krug, Stony Brook University,
Stony Brook, United States
10:00 – 10:15 (67) Pomalidamide
Restores Immune Recognition of Primary Effusion Lymphoma
Prabha Shrestha, HIV and AIDS Malignancy
Branch, Bethesda, United States
10:15 – 10:30 (68) Differences
in KSHV Seropositivity in a Cohort of Children in Western Kenya from Different
Malaria Transmission Regions
Katherine Sabourin, Colorado School of
Public Health, Aurora, United States
10:30 – 10:45 (69) KSHV viral
load in blood and saliva and cellular immune responses in healthy individuals
of a wide age range in rural Uganda
Angela Nalwoga, MRC/UVRI and LSHTM
Uganda Research Unit, Entebbe, Uganda
10:45 – 11:00 AM Coffee Break
Stuyvesant Ballroom Foyer
11:00 – 1:00 PM Session X. Bench to Bedside
Stuyvesant Ballroom
Session Chairs
Warren Phipps, Fred Hutchinson Cancer Research Center, USA
Charles Wood, Director and Professor, University of Nebraska, USA
Presentations
11:00 – 11:15 AM (70) Primary Role
of KSHV in Pathogenesis of Endemic and Epidemic Kaposi’s Sarcoma
Salum Lidenge,
University of Nebraska-Lincoln, Lincoln, United
States
11:15 – 11:30 AM (71) Physical
Barriers to Health Care Access and All-Cause Mortality among People Living with
AIDS-associated Kaposi Sarcoma from the Strategies to Improve Kaposi Sarcoma
Outcomes in Zimbabwe (SIKO) Study
Katherine Sabourin, Colorado School of
Public Health, Aurora, United States
11:30 – 11:45 AM (72) Utility of
Cerebral Spinal Fluid KSHV Viral Load in Primary Effusion Lymphoma
Kathryn Lurain, National Cancer Institute,
Bethesda, United
States
11:45 – 12:00 PM (73) Interrogating
Intra-host Diversity of KSHV
Clement Santiago, University of
Washington, Seattle, United States
12:00 – 12:15 PM (74) Treatment of
symptomatic Kaposi sarcoma herpesvirus-associated multicentric Castleman
disease (KSHV-MCD) with tocilizumab: Response and cytokine changes
Ramya Ramaswami,
National Cancer Institute, Bethesda, United
States
12:15 – 12:30 PM (75) Fragment-based
Discovery of a Qualified Hit Targeting the Latency-associated Nuclear Antigen
of the Oncogenic Kaposi’s Sarcoma-associated Herpesvirus/Human Herpesvirus 8
Saskia Stein, Hannover Medical School,
Hannover, Germany
12:30 – 12:45 PM (76) Delivery of
locked nucleic acid to specifically suppress herpesvirus miRNAs for primary
effusion lymphoma therapy
Enguo Ju,
University of Pittsburgh, Pittsburgh, United
States
12:45 – 01:00 PM (77) Modulating
vFLIP levels and NF-kB signaling using NEMO Coiled Coil Mimics
Jouliana Sadek, Weill Cornell Medicine,
New York, United
States
1:00 PM Adjourn